The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 18, 2025
Filed:
Jul. 31, 2021
Indian Oil Corporation Limited, Mumbai, IN;
Department of Biotechnology, New Delhi, IN;
Mukund Adsul, Faridabad, IN;
Simranjeet Kaur Sandhu, Faridabad, IN;
Reeta Rani Singhania, Faridabad, IN;
Anshu Shankar Mathur, Faridabad, IN;
Ravi Prakash Gupta, Faridabad, IN;
Deepak Kumar Tuli, Faridabad, IN;
Suresh Kumar Puri, Faridabad, IN;
Sankara Sri Venkata Ramakumar, Faridabad, IN;
INDIAN OIL CORPORATION LIMITED, Mumbai, IN;
Abstract
The present invention relates to a process for simultaneous production of citric acid and cellulolytic enzymes. The batch process comprising (i) adding slurry of a pre-treated lignocellulosic biomass or cellulose in a fermentation media; (ii) inoculating 10% (v/v) active liquid seed culture ofMRJ-16 in the fermentation media of step (i); (iii) subjecting the culture of step (ii) to fermentation in an aerated fermenter at a temperature of 28-33° C. for a duration of 96 hours; and (iv) collecting enzyme broth after fermentation of step (iii) to obtain citric acid and cellulolytic enzymes. The batch and fed-batch process of the present invention results in high titer production of citric acid and cellulolytic enzymes in a single step and using single microbial strain.