The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 18, 2025
Filed:
Jul. 19, 2017
Applicant:
Accellta Ltd., Haifa, IL;
Inventors:
Michal Amit, Misgav, IL;
Itzchak Angel, Nes Ziona, IL;
Assignee:
Accellta Ltd., Haifa, IL;
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07K 14/81 (2006.01); C12N 5/00 (2006.01); C12N 5/0735 (2010.01); C12N 5/074 (2010.01); C12N 5/18 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0606 (2013.01); C07K 14/81 (2013.01); C12N 5/0018 (2013.01); C12N 5/0031 (2013.01); C12N 5/0696 (2013.01); C12N 2500/99 (2013.01); C12N 2501/115 (2013.01); C12N 2501/2306 (2013.01); C12N 2501/415 (2013.01); C12N 2533/52 (2013.01); C12N 2533/90 (2013.01);
Abstract
Provided are defined culture media for culturing pluripotent stem cells in a suspension culture devoid of substrate adherence, the defined culture media comprising an effective amount of a protease inhibitor; a GSK3β inhibitor and at least one agent selected from the group consisting of a protease inhibitor and a WNT3A polypeptide; a WNT3A polypeptide and a stabilizing agent thereof with the proviso that said stabilizing agent is not a lipid vesicle; and/or a GSK3β inhibitor, with the proviso that the medium is devoid of an ERK1/2 inhibitor. Also provided are cell cultures and methods of suing same.