The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 11, 2025

Filed:

Sep. 23, 2020
Applicant:

Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US);

Inventors:

Robert Stairs, High Springs, FL (US);

James Reilly, Putnam Valley, NY (US);

John Mattila, Nyack, NY (US);

Samantha Wadsworth, Stamford, CT (US);

Assignee:

Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01D 15/20 (2006.01); B01D 15/32 (2006.01); B01J 20/34 (2006.01); C07K 1/20 (2006.01); C07K 16/06 (2006.01);
U.S. Cl.
CPC ...
B01J 20/3475 (2013.01); B01D 15/203 (2013.01); B01D 15/327 (2013.01); C07K 1/20 (2013.01); C07K 16/065 (2013.01); B01J 2220/58 (2013.01);
Abstract

Aspects of the present disclosure relate to a method of regenerating a hydrophobic interaction chromatography column to which a load mass has been applied, the method comprising passing one or more column volumes of an alkaline solution through hydrophobic interaction media within the column, wherein the alkaline solution exhibits a pH of between about 10 and about 14, and a conductivity of between 0.5 mS/cm and about 10 mS/cm, wherein material bound to the hydrophobic interaction media is removed. In some cases, the alkaline solution may include sodium hydroxide at a concentration of between, e.g., about 0.1 mM and 10 mM.


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