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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 12241061 B1

PDFFull Text
Date of Patent:
Mar. 04, 2025

Filed:

Feb. 04, 2020

Car library and scfv manufacturing method

Applicant:

National University Corporation Ehime University, Matsuyama, JP;

Inventors:

Toshiki Ochi, Ehime, JP;

Masaki Yasukawa, Ehime, JP;

Hiroshi Fujiwara, Ehime, JP;

Katsuto Takenaka, Ehime, JP;

Assignee:

National University Corporation Ehime University, Ehime, JP;

Attorney:

HSML P.C.

Primary Examiner:

Ilia I Ouspenski

Int. Cl.
CPC ...
C12N 15/10 (2006.01); A61K 39/00 (2006.01); C07K 14/47 (2006.01); C07K 14/705 (2006.01); C07K 14/725 (2006.01); C07K 16/28 (2006.01); C12N 5/0783 (2010.01); C12N 15/62 (2006.01); C40B 40/08 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1068 (2013.01); A61K 39/4611 (2023.05); A61K 39/4631 (2023.05); A61K 39/464412 (2023.05); A61K 39/464488 (2023.05); C07K 14/7051 (2013.01); C07K 14/70521 (2013.01); C07K 16/2803 (2013.01); C12N 5/0636 (2013.01); A61K 2239/31 (2023.05); A61K 2239/38 (2023.05); A61K 2239/48 (2023.05); C07K 2317/14 (2013.01); C07K 2317/565 (2013.01); C07K 2317/622 (2013.01); C07K 2319/02 (2013.01); C07K 2319/03 (2013.01); C07K 2319/30 (2013.01); C07K 2319/33 (2013.01);
Abstract

Provided are a CAR library used to screen scFvs that can be functional in CAR-T cells, and an scFv manufacturing method in which the CAR library is used. A chimeric antigen receptor (CAR) library of the present invention includes nucleic acids coding for first CARs. Each of the first CARs includes a first antigen-binding domain, a first transmembrane domain, and a first intracellular signaling domain. The first antigen-binding domain includes a first single-chain antibody (scFv) to be screened for the ability to bind to a target antigen. The first scFv includes a first heavy-chain variable region and a first light-chain variable region. The first heavy-chain variable region and the first light-chain variable region meet a predetermined condition.

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