Molecular marker significantly associated with vitamin e content in soybeans, kompetitive allele specific polymerase chain reaction primers combination and application thereof

Abstract

A molecular marker significantly associated with vitamin E content in soybeans, a Kompetitive Allele-Specific polymerase chain reaction (KASP) primers combination and an application thereof are provided in the present disclosure, belonging to the field of molecular genetics and breeding. A nucleotide sequence of the molecular marker is shown in SEQ ID NO. 1, and there is an A/T mutation at the 21st base. The KASP primer set for detecting the molecular marker includes an upstream primer F1 with a nucleotide sequence as shown in SEQ ID NO. 2, an upstream primer F2 with a nucleotide sequence as shown in SEQ ID NO. 3 and a downstream primer r with a nucleotide sequence as shown in SEQ ID NO. 4. The KASP primers combination developed by the present disclosure accurately distinguish soybeans with high and low vitamin E content.