The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 25, 2025
Filed:
Aug. 29, 2019
Mizuho Medy Co., Ltd., Saga, JP;
Takashi Nagano, Saga, JP;
Koichi Ebisu, Saga, JP;
Kenji Narahara, Saga, JP;
Kazuhiro Ichimaru, Saga, JP;
MIZUHO MEDY CO., LTD., Saga, JP;
Abstract
The present invention provides a method for detecting multiple target nucleic acids, the method making it possible to amplify a plurality of genes using one reaction vessel containing therein one type of reaction solution and further using a single label. Computation is performed in accordance with the following formulas for every specific cycle and/or every cycle during amplification reaction using a kit for detecting the multiple target nucleic acids. (Formula 1): f1[n]=fhyb.1[n]/fden.1[n], (Formula 1'): f2[n]=fhyb.2[n]/fden.2[n], (Formula 2): Fr[n]=(a−f2 [n])/(a−f1 [n]).fhyb.1[n]: Fluorescence intensity value in elongation step of first target nucleic acid detecting step. The same hereinafter.