The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 25, 2025

Filed:

Apr. 06, 2020
Applicant:

Life Technologies Corporation, Carlsbad, CA (US);

Inventors:

Wolfgang Hinz, Carlsbad, CA (US);

John Leamon, Stonington, CT (US);

David Light, Branford, CT (US);

Jonathan M. Rothberg, Guilford, CT (US);

Assignee:

Life Technologies Corporation, Carlsbad, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); B01J 19/00 (2006.01); C12Q 1/6874 (2018.01); C40B 40/08 (2006.01); C40B 50/06 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1093 (2013.01); B01J 19/0046 (2013.01); C12Q 1/6874 (2013.01); C40B 40/08 (2013.01); C40B 50/06 (2013.01); B01J 2219/00466 (2013.01); B01J 2219/005 (2013.01); B01J 2219/00596 (2013.01); B01J 2219/00722 (2013.01); Y10T 428/2982 (2015.01);
Abstract

The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.


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