The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 25, 2025
Filed:
Aug. 04, 2021
City University of Hong Kong, Hong Kong, CN;
Dong Sun, Hong Kong, CN;
Fei Pan, Hong Kong, CN;
Shuxun Chen, Hong Kong, CN;
Yang Jiao, Hong Kong, CN;
City University of Hong Kong, Hong Kong, CN;
Abstract
Two or more micropipettes are used to increase a microinjection throughput in an automated system for microinjecting adherent cells on a Petri dish. In the system, a motorized stage carrying the Petri dish sequentially visits the cells according to an optimized injection sequence. The sequence is selected by minimizing a total distance traveled by the motorized stage such that each cell is visited once by one of the micropipettes. Using multiple micropipettes advantageously reduces the minimized total distance over using a single micropipette to thereby increase the throughput. The optimized injection sequence is obtained by solving an equality-generalized traveling salesman problem. Each micropipette is mounted on a motorized micromanipulator. The motorized stage and motorized micromanipulators operate coordinately that each micromanipulator goes down or up during movement of the motorized stage to compensate for unevenness between a focus plane and a moving trajectory of the motorized stage.