The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 18, 2025

Filed:

Jan. 04, 2019
Applicant:

Toyo Seikan Group Holdings, Ltd., Tokyo, JP;

Inventors:

Ryo Suenaga, Yokohama, JP;

Maki Mitsuishi, Yokohama, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12M 1/00 (2006.01); C12M 3/06 (2006.01);
U.S. Cl.
CPC ...
C12M 23/14 (2013.01); C12M 23/50 (2013.01); C12M 27/16 (2013.01); C12M 29/26 (2013.01); C12N 5/0068 (2013.01); C12N 2513/00 (2013.01); C12N 2527/00 (2013.01);
Abstract

A cell culturing method and device that uses a cell culture bag () that includes a bag body () configured from an upper surface film () and a lower surface film () having a sealed periphery, and a port () attached to the bag body (), and a plurality of recesses () being formed in the lower surface film (). The method including: a closing-off step for closing off the plurality of recesses () using the upper surface film () by discharging a culture medium (S) contained in the bag body () through the port (); and a releasing step for releasing, in some or all of the plurality of closed-off recesses (), cells or cell aggregates adhering to the inner surfaces of the recesses ().


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