The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 11, 2025
Filed:
Feb. 03, 2023
Applicant:
The Trustess of Columbia University IN the City of New York, New York, NY (US);
Inventor:
Maxwell Gottesman, New York, NY (US);
Assignee:
University in the City of New York, New York, NY (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/00 (2006.01); C07K 14/245 (2006.01); C12N 9/52 (2006.01); C12N 15/10 (2006.01); C12N 15/70 (2006.01); C12P 21/02 (2006.01);
U.S. Cl.
CPC ...
C12P 21/02 (2013.01); C07K 14/245 (2013.01); C12N 9/52 (2013.01); C12N 15/102 (2013.01); C12N 15/70 (2013.01);
Abstract
The present disclosure provides host cells for reliable, high yield recombinant protein production, including unstable proteins. The present host cell (e.g., a bacterial cell) is deficient in at least one protease (or a subunit of a protease) such as Clp or ClpP. The host cell may also contain an expression vector that encodes a protein or polypeptide for overexpression.