The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 04, 2025
Filed:
Jun. 22, 2021
Amazon Technologies, Inc., Seattle, WA (US);
Miro Yakov Shverdin, Mountain View, CA (US);
Annapurna Karicherla, Cupertino, CA (US);
Carla Alejandra Gimenez, Buenos Aires, AR;
Amazon Technologies, Inc., Seattle, WA (US);
Abstract
Systems and methods for testing a biological sample for each of two or more target pathogens employ fluorescence detection from a single fluorescence reading chamber. A method of testing a biological sample for each of two or more target pathogens includes forming a detection cycle liquid by combining detection cycle compounds with a biological sample liquid formed from a biological sample. The detection cycle compounds include respective CRISPR enzymes for each of the two or more target pathogens. The respective CRISPR enzymes are tagged with respective spectrally unique fluorescent probes. The detection cycle liquid is exposed to a multi-wavelength fluorescence excitation light. A multi-wavelength fluorescence light emission detection signal indicative of intensity of fluorescence light emitted by the detection cycle liquid over multiple wavelengths is processed to generate detection data indicative of presence or absence of each of the two or more target pathogens in the biological sample.