The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 28, 2025
Filed:
May. 11, 2021
Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
Illumina, Inc., San Diego, CA (US);
Illumina Singapore Pte. Ltd., Singapore, SG;
Saurabh Rajendra Nirantar, Singapore, SG;
Sergio Peisajovich, San Diego, CA (US);
Eric Brustad, San Diego, CA (US);
Illumina, Inc., San Diego, CA (US);
Illumina Singapore Pte. Ltd., Singapore, SG;
Abstract
Disclosed herein include methods of generating a single stranded deoxyribonucleic acid (ssDNA) scaffold comprising nucleotides with modified bases using a recombinant terminal deoxynucleotidyl transferase (TdT). The recombinant TdT can comprise an amino acid sequence that is at least 80% identical to aTdT, or a fragment thereof, and for example comprise one or more amino acid substitution mutations at one or more positions functionally equivalent to Glu191, Lys193, Glu194, Asp242, Lys287, Phe296, Met299, Thr342, and His420 in theTdT.