The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 21, 2025
Filed:
Mar. 15, 2018
The Broad Institute, Inc., Cambridge, MA (US);
Massachusetts Institute of Technology, Cambridge, MA (US);
President and Fellows of Harvard College, Cambridge, MA (US);
Omar Abudayyeh, Cambridge, MA (US);
James Joseph Collins, Cambridge, MA (US);
Jonathan Gootenberg, Cambridge, MA (US);
Feng Zhang, Cambridge, MA (US);
Eric S. Lander, Cambridge, MA (US);
Pardis Sabeti, Cambridge, MA (US);
Catherine Amanda Freije, Cambridge, MA (US);
Cameron Myhrvold, Cambridge, MA (US);
THE BROAD INSTITUTE, INC., Cambridge, MA (US);
MASSACHUSETTS INSTITUTE OF TECHNOLOGY, Cambridge, MA (US);
PRESIDENT AND FELLOWS OF HARVARD COLLEGE, Cambridge, MA (US);
Abstract
Provided herein is a nucleic acid detection system comprising: a CRISPR system comprising an effector protein and one or more guide RNAs designed to bind to corresponding target molecules; an RNA-based masking construct; and optionally, nucleic acid amplification reagents to amplify target RNA molecules in a sample. In another aspect, the embodiments provide a polypeptide detection system comprising: a CRISPR system comprising an effector protein and one or more guide RNAs designed to bind a trigger RNA, an RNA-based masking construct; and one or more detection aptamers comprising a masked RNA polymerase promoter binding site or a masked primer binding site. In some embodiments, the system may be used to detect viruses in samples.