The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 21, 2025
Filed:
Feb. 01, 2024
Guangzhou Geneseed Biotech Co., Ltd., Guangzhou, CN;
Ming Liu, Guangzhou, CN;
Maolei Zhang, Guangzhou, CN;
Yesheng Wang, Guangzhou, CN;
Qiujie Cai, Guangzhou, CN;
Wanjun Zhang, Guangzhou, CN;
Xiaodan Ma, Guangzhou, CN;
Guangzhou Geneseed Biotech Co., Ltd., Guangzhou, CN;
Abstract
The present invention relates to the technical field of nucleic acids, and in particular, to a method for precisely preparing a circular RNA with anintron self-cleaving ribozyme. By redesigning a target nucleic acid sequence of a linear RNA, selecting a site suitable for precise cleavage by the intron self-cleaving ribozyme, and removing exon sequences from theintron self-cleaving ribozyme, precise cyclization of RNAs after in vitro transcription is achieved. The method provided by the present invention solves the problem in the prior art that linear RNAs cannot be precisely cyclized during the process of generating circular RNAs in vitro, and provides an ideal in vitro preparation method for circular RNA applications.