The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 21, 2025
Filed:
Apr. 30, 2024
Anhui Agricultural University, Hefei, CN;
Yingshang Shili Ecological Agriculture Technology Co., Ltd., Fuyang, CN;
Guohu Chen, Hefei, CN;
Qian Yin, Hefei, CN;
Ting Li, Hefei, CN;
Chenggang Wang, Hefei, CN;
Xiaoyan Tang, Hefei, CN;
Ying Wang, Hefei, CN;
Xueqing Liu, Hefei, CN;
Hongwei Wen, Hefei, CN;
Siwen Wu, Hefei, CN;
Anhui Agricultural University, Hefei, CN;
Yingshang Shili Ecological Agriculture Technology Co., Ltd., Fuyang, CN;
Abstract
A method for obtaining regenerated seedlings ofL. ssp.from embryonic tip tissues, including the following steps. A seed is inoculated to a germination medium for dark culture for 60 h. The testa, root tip, two cotyledons and middle growing point of the resultant germinated seed are removed, and an embryonic tip with a length of 3-5 mm is retained as an explant. The explant is sequentially subjected to low-temperature pre-culture in a pre-culture medium for 36 h, room-temperature shaking culture in a liquid bud induction culture medium for 10 min, and bud induction culture in a bud induction culture medium for 20 d. A regenerated plant with 5-6 leaves is transferred to a rooting medium for rooting culture for about 2 weeks, and a well-rooted plant is collected, and subjected to hardening and transplantation into a filed.