The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 14, 2025

Filed:

Mar. 28, 2018
Applicant:

Japan Science and Technology Agency, Kawaguchi, JP;

Inventors:

Hiroyuki Noji, Tokyo, JP;

Takao Ono, Osaka, JP;

Yoshihiro Minagawa, Tokyo, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 1/36 (2006.01); B01L 3/00 (2006.01); G01N 21/64 (2006.01); G01N 21/78 (2006.01); G01N 33/569 (2006.01); G01N 33/58 (2006.01);
U.S. Cl.
CPC ...
G01N 1/36 (2013.01); B01L 3/502 (2013.01); G01N 21/6428 (2013.01); G01N 21/78 (2013.01); G01N 33/56983 (2013.01); G01N 33/581 (2013.01); B01L 2200/142 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0858 (2013.01); B01L 2300/0864 (2013.01);
Abstract

There is provided a method of detecting a microscopic body stored in a plurality of receptacles formed separately from each other. The method, which is provided as a technique for enclosing a to-be-detected substance such as nucleic acid, protein, virus, and cell by means of a simple operation in droplets of an extremely small volume and enabling highly sensitive detection, includes the steps of (1) introducing a solvent into a space between a lower layer part in which the receptacles are formed and an upper layer part facing a surface of the lower layer part in which surface the receptacles are formed, wherein the solvent contains the microscopic body; (2) introducing gas into the space to form a droplet of the solvent in the receptacles, wherein the droplet contains the microscopic body; and (3) detecting the microscopic body present in the droplet optically, electrically, and/or magnetically.


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