The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 14, 2025
Filed:
Feb. 11, 2021
Applicant:
Saint Louis University, St. Louis, MO (US);
Inventors:
Xiaofeng Fan, St. Louis, MO (US);
Adrian Michael Di Bisceglie, St. Louis, MO (US);
Assignee:
Saint Louis University, St. Louis, MO (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6848 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6848 (2013.01); C12Q 1/6806 (2013.01); C12Q 2521/319 (2013.01); C12Q 2525/125 (2013.01); C12Q 2531/107 (2013.01); C12Q 2531/125 (2013.01);
Abstract
Provided herein are methods of preparing a template nucleic acid for next-generation sequencing. The methods comprise performing primer extension using cdGTP instead of dGTP, which then allows for the input nucleic acids to be selectively digested by cdGTP-resistant restriction enzymes, thereby enriching the template nucleic acid prior to next-generation sequencing library preparation.