The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 14, 2025
Filed:
Jun. 14, 2022
Massachusetts Institute of Technology, Cambridge, MA (US);
The Broad Institute, Inc., Cambridge, MA (US);
President and Fellows of Harvard College, Cambridge, MA (US);
Kaiyi Jiang, Cambridge, MA (US);
Rohan Neil Krajeski, Boston, MA (US);
Omar Osama Abudayyeh, Cambridge, MA (US);
Jonathan S. Gootenberg, Cambridge, MA (US);
Yifan Zhang, Cambridge, MA (US);
Fei Chen, Cambridge, MA (US);
Xi Chen, Roxbury Crossing, MA (US);
Jeremy G. Koob, Somerville, MA (US);
Massachusetts Institute of Technology, Cambridge, MA (US);
The Broad Institute, Inc., Cambridge, MA (US);
President and Fellows of Harvard College, Cambridge, MA (US);
Abstract
RNA editing tools for use in systems designed to measure RNA in vivo and manipulate specific cell types are disclosed herein. An RNA sensor system comprising a) a single-stranded RNA (ssRNA) sensor comprising a stop codon and a payload; optionally wherein the ssRNA sensor further comprises a normalizing gene; and b) an adenosine deaminase acting on RNA (ADAR) deaminase; wherein the sensor is capable of binding to a ssRNA target to form a double-stranded RNA (dsRNA) duplex that becomes a substrate for the ADAR deaminase; wherein the substrate comprises a mispairing within the stop codon; and wherein the mispairing is editable by the ADAR deaminase, which editing can effectively remove the stop codon so as to enable translation and expression of the payload. A method of quantifying ribonucleic acid (RNA) levels using the RNA sensor system is also disclosed.