The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 07, 2025
Filed:
Sep. 27, 2021
Applicant:
The University of Chicago, Chicago, IL (US);
Inventors:
Chuan He, Chicago, IL (US);
Boxuan Simen Zhao, Chicago, IL (US);
Pradnya Narkhede, Chicago, IL (US);
Chang Liu, Chicago, IL (US);
Xiaolong Cui, Chicago, IL (US);
Assignee:
The University of Chicago, Chicago, IL (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6844 (2018.01); C12Q 1/6806 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6844 (2013.01); C12Q 1/6806 (2013.01);
Abstract
Methods, compositions and kits are provided to amplify an amount of genomic methylated DNA that can be subsequently analyzed and/or sequenced. It has particular use with small amounts of DNA, including, but not limited to cell free DNA samples. In some embodiments, the ratio of polymerase and methyltransferase is controlled in order to provide maximum yields. In some embodiments, a dual primase/polymerase is used.