The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 07, 2025

Filed:

Feb. 07, 2024
Applicant:

Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing, CN;

Inventors:

Jie Du, Beijing, CN;

Yuan Wang, Beijing, CN;

Fengjuan Li, Beijing, CN;

Xin Tan, Beijing, CN;

Xue Wang, Beijing, CN;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/115 (2010.01); A61K 31/7088 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
C12N 15/115 (2013.01); A61K 31/7088 (2013.01); G01N 33/6893 (2013.01); C12N 2310/16 (2013.01); C12N 2320/30 (2013.01); G01N 2800/321 (2013.01); G01N 2800/323 (2013.01); G01N 2800/324 (2013.01); G01N 2800/325 (2013.01); G01N 2800/52 (2013.01);
Abstract

A nucleic acid aptamer specifically recognizing soluble ST2 protein and its application, belonging to the field of variation or genetic engineering, are described. The technical problem addressed includes how to specifically detect sST2 protein. In order to address this technical problem, nucleic acid aptamers of single-stranded DNA with nucleotide sequences are shown in SEQ ID No. 1-10, respectively. SELEX technology, combined with high-throughput sequencing technology and bioinformatics analysis, reduces the rounds of screening and obtains candidate nucleic acid aptamers, and further analyzes its affinity and specificity to obtain a nucleic acid aptamer that specifically recognizes sST2 protein. The nucleic acid aptamer has the characteristics of high specificity, high stability, convenient synthesis, and easy labeling of functional groups, and the like, and may be used for the detection of sST2 protein and the preparation of biosensors, diagnosis and prognosis of cardiovascular diseases and other products.


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