The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 31, 2024
Filed:
Mar. 13, 2023
Applicant:
Ventana Medical Systems, Inc., Tucson, AZ (US);
Inventors:
Assignee:
Ventana Medical Systems, Inc., Tucson, AZ (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 1/30 (2006.01); G01N 1/31 (2006.01); G01N 29/024 (2006.01); G01N 29/07 (2006.01); G01N 29/44 (2006.01);
U.S. Cl.
CPC ...
G01N 1/30 (2013.01); G01N 1/31 (2013.01); G01N 29/024 (2013.01); G01N 29/07 (2013.01); G01N 29/4418 (2013.01); G01N 29/4427 (2013.01); G01N 29/4472 (2013.01); G01N 2001/305 (2013.01); G01N 2291/011 (2013.01); G01N 2291/023 (2013.01); G01N 2291/02475 (2013.01); G01N 2291/02809 (2013.01);
Abstract
A method is disclosed that permits calculation of reagent concentrations (in SI units) over time and space within a tissue sample as the sample is immersed in the reagent and the reagent diffuses into the tissue sample. The disclosed method has yielded the surprising result that once a formaldehyde concentration at all points within a tissue sample exceeds about 90 mM during a cold step of a cold+hot fixation protocol, the hot step of the fixation protocol can be commenced to provide reliable detection of molecular targets and preservation of tissue morphology in downstream analyses.