The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 31, 2024

Filed:

Dec. 20, 2021
Applicant:

Winfield Solutions, Llc, Shoreview, MN (US);

Inventors:

Jonathan Zuk, Hayfield, MN (US);

Stephanie Wedryk, Shoreview, MN (US);

Assignee:

WinField Solutions, LLC, Arden Hills, MN (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12Q 1/6874 (2018.01); C12Q 1/6895 (2018.01);
U.S. Cl.
CPC ...
C12N 15/1003 (2013.01); C12Q 1/6874 (2013.01); C12Q 1/6895 (2013.01); C12Q 2600/158 (2013.01);
Abstract

Methods of extracting ribonucleic acid from plant tissue involve treating multiple groups of plant seeds with different plant growth regulators. Each group of plant seeds can be treated with a different plant growth regulator. Methods further involve germinating the plant seeds to form seedlings, harvesting tissue samples from subgroups within each group of plant seeds and seedlings at different timepoints, and extracting total RNA from each harvested tissue sample. Methods can also involve sequencing the extracted RNA, determining an expression level of a plurality of genes relative to a negative control, and identifying a subset of differentially expressed genes at each distinct timepoint.


Find Patent Forward Citations

Loading…