The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 31, 2024

Filed:

Feb. 25, 2019
Applicant:

Boise State University, Boise, ID (US);

Inventors:

John Everingham, Boise, ID (US);

Trevor Lujan, Boise, ID (US);

Assignee:

Boise State University, Boise, ID (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/00 (2006.01); B01L 3/00 (2006.01); C12M 3/00 (2006.01); C12N 5/00 (2006.01);
U.S. Cl.
CPC ...
C12M 23/34 (2013.01); B01L 3/502707 (2013.01); B01L 3/505 (2013.01); C12M 21/08 (2013.01); C12N 5/0068 (2013.01); C12N 2527/00 (2013.01); C12N 2533/40 (2013.01); C12N 2535/00 (2013.01); C12N 2539/00 (2013.01);
Abstract

A mold capable of preparing a cellular or acellular construct includes a block including a three-dimensional cavity for a sample, a base, and a removable sheet inserted between the block and the base. The sheet may be coated with silicone grease prior to insertion of the sheet between the block and the base. Mechanical constraints may be included at each end of the cavity to constrain cell driven contraction and to allow for gripping the sample after the sample is removed from the mold. The sample may be prepared by partially filling the cavity with gel, filling the remaining portion of the cavity with culture media, and allowing a three-dimensional sample to culture within the cavity for a time. The sample may be transported to a bioreactor and ejected from the mold by removing the sheet and applying pressure to the mechanical constraints.


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