The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 24, 2024
Filed:
Jan. 31, 2019
Sansure Biotech Inc., Hunan, CN;
Lizhong Dai, Changsha, CN;
Xu Fan, Changsha, CN;
SANSURE BIOTECH INC., Hunan, CN;
Abstract
A rapid amplification method for a nucleic acid of hepatitis B virus, comprises the following steps: mixing a sample containing hepatitis B virus with a nucleic acid releasing agent, and adding a PCR premix to obtain a reaction solution, the PCR premix comprising deoxyribonucleoside triphosphate, an upstream primer as shown in the sequence SEQ ID NO: 1, a downstream primer as shown in the sequence SEQ ID NO: 2, a DNA polymerase and an amplification buffer; placing the reaction solution in a PCR reaction tube so that the reaction solution is in a form of a thin film with a thickness of 0.1 mm or less; performing PCR amplification under the following reaction condition: pre-denaturation at 90 to 100° C. for 10 s to 600 s, denaturation at 90 to 100° C. for 0 to 1 s, and annealing and extending at 50-65° C. for 0 to 1 s.