The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 24, 2024

Filed:

Jul. 26, 2023
Applicant:

Shandong Computer Science Center (National Supercomputer Center IN Jinan), Shandong, CN;

Inventors:

Xin Wang, Jinan, CN;

Ming Yang, Jinan, CN;

Xiaoming Wu, Jinan, CN;

Fuqiang Wang, Jinan, CN;

Yan Liang, Jinan, CN;

Zhenya Chen, Jinan, CN;

Chao Mu, Jinan, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/10 (2006.01); G16B 5/00 (2019.01);
U.S. Cl.
CPC ...
C12N 9/1096 (2013.01); C12Y 206/01021 (2013.01); G16B 5/00 (2019.02);
Abstract

A new mutant of D-amino acid transaminase, based on supercomputing-assisted technology, belongs to the fields of computational biology, computer-aided design, and enzyme engineering technology, and specifically relates to mutants of D-amino acid transaminase obtained based on supercomputing-assisted technology and applications thereof. Compared to the wild-type enzyme, the aforementioned mutant of D-amino acid transaminase exhibits a half-life (t) of over 12 hours at 40° C., while it is only 8.8 minutes for the wild-type D-amino acid transaminase. The half-inactivation temperature (T) of the mutant is 45.3° C., approximately 5.4° C. higher than that of the wild-type D-amino acid transaminase. This substantially enhances its thermal stability, enzymatic activity, etc., effectively broadening its application fields and scope. The mutant has a wide range of industrial applications and thus holds substantial practical value.


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