The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 24, 2024

Filed:

Jun. 26, 2020
Applicants:

Crispr Therapeutics Ag, Zug, CH;

Bayer Healthcare Llc, Whippany, NJ (US);

Inventors:

Ryo Takeuchi, Cambridge, MA (US);

Abraham Scaria, Cambridge, MA (US);

Assignees:

CRISPR THERAPEUTICS AG, Zug, CH;

BAYER HEALTHCARE LLC, Whippany, NJ (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/11 (2006.01); C12N 9/22 (2006.01); C12N 15/64 (2006.01); C12N 15/86 (2006.01);
U.S. Cl.
CPC ...
C12N 15/64 (2013.01); C12N 9/22 (2013.01); C12N 15/86 (2013.01); C12N 2310/122 (2013.01); C12N 2310/20 (2017.05); C12N 2750/14143 (2013.01); C12N 2750/14151 (2013.01); C12N 2800/22 (2013.01);
Abstract

The present application provides a CRISPR/Cas system comprising a nuclease segment that encodes a Cas9 nuclease or variant thereof, a guide RNA segment comprising a nucleotide sequence that encodes a gRNA or sgRNA, and a promoter segment comprising a nucleotide sequence that encodes a first promoter comprising one or more tetracycline operator sequence, wherein the gRNA segment is operably linked to the promoter segment. The present application also provides materials and methods for controlling transcriptional expression of guide RNAs and/or post-transcriptional expression of Cas nuclease.


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