The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 24, 2024

Filed:

Jan. 24, 2024
Applicant:

Molecular Devices, Llc, San Jose, CA (US);

Inventors:

Timothy A. Floto, Scotts Valley, CA (US);

Alison A. Glaser, Philadelphia, PA (US);

Lee Von Landau, Hampshire, GB;

John Phillips, San Jose, CA (US);

Kyle Corey, San Jose, CA (US);

Padmavathi Bandhuvula, Fremont, CA (US);

Anna Louise Forsyth, Sunnyvale, CA (US);

Assignee:

Molecular Devices, LLC, San Jose, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/34 (2006.01); C12M 1/00 (2006.01); C12M 1/26 (2006.01); C12M 1/36 (2006.01); G01N 21/17 (2006.01); G01N 21/64 (2006.01); G06T 7/00 (2017.01); G06T 15/00 (2011.01); G06T 15/08 (2011.01); G01N 15/1434 (2024.01);
U.S. Cl.
CPC ...
C12M 41/36 (2013.01); C12M 1/26 (2013.01); C12M 1/34 (2013.01); C12M 41/48 (2013.01); C12M 47/04 (2013.01); G01N 21/17 (2013.01); G01N 21/6452 (2013.01); G01N 21/6456 (2013.01); G06T 7/00 (2013.01); G06T 7/0012 (2013.01); G06T 7/0016 (2013.01); G06T 15/00 (2013.01); G06T 15/08 (2013.01); G01N 2015/1445 (2013.01); G01N 2021/1782 (2013.01);
Abstract

Systems, including methods and apparatus, for growing viable monoclonal colonies, identifying them as monoclonal after their viability has been demonstrated, and picking and placing them into a target environment. The methods may include plating cells at low density in a well, capturing at a first time shortly after plating a series of vertically spaced-apart images of the well showing locations of the cells, capturing at a second, later time images of the same well showing locations of candidate cell colonies, determining a likelihood that the candidate colonies are monoclonal based on information in the first set of images, and, based on the likelihood, picking candidate colonies using a picking head for transfer to a target environment.


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