The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 17, 2024

Filed:

Dec. 18, 2017
Applicants:

Nippn Corporation, Tokyo, JP;

Fasmac Co., Ltd., Atsugi, JP;

Inventors:

Mika Tsuna, Atsugi, JP;

Yoshitaka Harada, Atsugi, JP;

Yasuhiro Seto, Atsugi, JP;

Kazuto Takasaki, Atsugi, JP;

Assignees:

NIPPN CORPORATION, Tokyo, JP;

FASMAC CO., LTD., Atsugi, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/689 (2018.01); C12Q 1/6853 (2018.01); C12Q 1/686 (2018.01); C12Q 1/6869 (2018.01); G01N 33/52 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/689 (2013.01); C12Q 1/6853 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6869 (2013.01); G01N 33/52 (2013.01); C12Q 2600/156 (2013.01); G01N 2458/00 (2013.01); G01N 2469/00 (2013.01);
Abstract

Novel means that enables detection of thebacterium alone distinctly from other bacteria belonging to the genuswith sufficiently high accuracy is disclosed. The present inventors intensively analyzed the genome of thebacterium to identify two genes (the lmo0084 gene and the lmo2736 gene) as target regions with which thebacterium can be specifically detected distinctly from other bacteria belonging to the genusutilizing a nucleic acid amplification method. By a further intensive study of the base sequences of these two genes, primer setting regions for highly accurate, specific detection of thebacterium alone were identified, and preferred particular examples of PCR primer sets, LAMP primer sets, and real-time PCR primer-probe sets were established.


Find Patent Forward Citations

Loading…