The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 17, 2024

Filed:

Aug. 30, 2018
Applicant:

Hitachi Chemical Diagnostics Systems Co., Ltd., Tokyo, JP;

Inventors:

Mizuki Miura, Shizuoka, JP;

Tomoko Aratake, Shizuoka, JP;

Kenta Kinjo, Shizuoka, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/60 (2006.01); C12Q 1/32 (2006.01); C12Q 1/44 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/60 (2013.01); C12Q 1/32 (2013.01); C12Q 1/44 (2013.01); G01N 2333/902 (2013.01); G01N 2333/918 (2013.01); G01N 2405/00 (2013.01);
Abstract

To provide a method for simply and precisely measuring cholesterol (LDL-C) in low-density lipoprotein in a sample without use of any surfactant having an alkylphenol structure, in terms of environmental friendliness. A method for measuring LDL-C in a sample, the method comprising: reacting the sample with (i) a combination of cholesterol ester hydrolase and cholesterol oxidase or (ii) a combination of cholesterol ester hydrolase, an oxidized coenzyme and cholesterol dehydrogenase, in an aqueous solvent which comprises: [a] one or more surfactants selected from the group consisting of a polyoxyethylene alkyl ether and a polyoxyethylene polyoxypropylene alkyl ether; and [b] a polyoxyethylene polyoxypropylene copolymer; and which does not comprise any surfactant having an alkylphenol structure; and measuring a substance formed or consumed in the reaction.


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