The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 17, 2024

Filed:

Mar. 03, 2020
Applicant:

Fenwal, Inc., Lake Zurich, IL (US);

Inventors:

Kyungyoon Min, Kildeer, IL (US);

Benjamin E. Kusters, Pleasant Prairie, WI (US);

Assignee:

Fenwal, Inc., Lake Zurich, IL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/078 (2010.01); A61K 35/17 (2015.01); A61M 1/02 (2006.01); A61M 1/34 (2006.01); A61M 1/36 (2006.01); A61M 1/38 (2006.01); C12M 1/00 (2006.01); C12M 1/42 (2006.01); C12N 5/00 (2006.01); C12N 5/0783 (2010.01); C12N 5/10 (2006.01); C12N 13/00 (2006.01); C12N 15/85 (2006.01);
U.S. Cl.
CPC ...
A61M 1/0281 (2013.01); A61M 1/0218 (2014.02); A61M 1/0231 (2014.02); A61M 1/362227 (2022.05); A61M 1/36224 (2022.05); A61M 1/36225 (2022.05); A61M 1/362261 (2022.05); A61M 1/362265 (2022.05); A61M 1/3696 (2014.02); C12M 35/02 (2013.01); C12M 45/05 (2013.01); C12N 5/0087 (2013.01); C12N 5/10 (2013.01); C12N 13/00 (2013.01); A61M 2202/0411 (2013.01); A61M 2205/3303 (2013.01); A61M 2206/16 (2013.01);
Abstract

Blood from a blood source is drawn into a fluid flow circuit. A mononuclear cell product is separated from the blood, followed by at least a portion of the mononuclear cell product being conveyed into an electroporation device without disconnecting the blood source from the fluid flow circuit. The electroporation device opens pores in a membrane of at least one of the cells of the mononuclear cell product to allow DNA material (which is added to the mononuclear cell product prior to electroporation) to enter and modify the genome of the cell. At least a portion of the modified mononuclear cell product is returned to the blood source. The mononuclear cell product may be washed prior to being conveyed into the electroporation device. The modified mononuclear cell product may be washed after exiting the electroporation device.


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