The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 10, 2024

Filed:

Jan. 22, 2021
Applicants:

The Broad Institute, Inc., Cambridge, MA (US);

Massachusetts Institute of Technology, Cambridge, MA (US);

Dana-farber Cancer Institute, Inc., Boston, MA (US);

Inventors:

Aviv Regev, Cambridge, MA (US);

Johanna Klughammer, Cambridge, MA (US);

Daniel Abravanel, Boston, MA (US);

Assignees:
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G16B 45/00 (2019.01); C12N 5/09 (2010.01); C12Q 1/6841 (2018.01); C12Q 1/6886 (2018.01); G01N 33/50 (2006.01); G16B 25/10 (2019.01); G16H 30/40 (2018.01); G16H 70/60 (2018.01);
U.S. Cl.
CPC ...
G16B 45/00 (2019.02); C12N 5/0693 (2013.01); C12Q 1/6841 (2013.01); C12Q 1/6886 (2013.01); G01N 33/5023 (2013.01); G16B 25/10 (2019.02); G16H 30/40 (2018.01); G16H 70/60 (2018.01); C12Q 2600/158 (2013.01);
Abstract

The present invention discloses novel methods and uses thereof for producing molecular spatial maps of metastatic breast cancer (MBC) and ductal carcinoma in situ of the breast (DCIS). A specific list of genes are identified using single-cell RNA sequencing and single-nucleus RNA sequencing and are used for RNA visualization of MBC and DCIS tissue microenvironment. Unexpected subtypes of tumor cells are revealed, and methods for identifying molecular biomarkers for MBC in the bone and breast and DCIS are disclosed. Furthermore, methods for identifying therapeutic agents and uses thereof for treating MBC and DCIS as well as compositions thereof comprising such identified therapeutic agents are provided.


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