The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 10, 2024
Filed:
Nov. 20, 2020
Applicant:
University of Washington, Seattle, WA (US);
Inventors:
Georg Seelig, Seattle, WA (US);
Anna Kuchina, Seattle, WA (US);
Leandra Brettner, Seattle, WA (US);
William DePaolo, Seattle, WA (US);
Assignee:
University of Washington, Seattle, WA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6874 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6874 (2013.01);
Abstract
Methods and kits for uniquely labeling nucleic acid molecules within a plurality of microbial cells are described. In an embodiment, the method comprises fixing and permeabilizing the plurality of microbial cells; dissociating microbial cell aggregates within a suspension comprising the plurality of microbial cells; reverse transcribing mRNA within the plurality of microbial cells to provide cDNA; and combinatorially labelling the cDNA to provide labelled cDNA.