The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 10, 2024

Filed:

Aug. 21, 2020
Applicant:

Monoquant Pty Ltd., Adelaide, AU;

Inventor:

Alexander Alan Morley, Toorak, AU;

Assignee:

Monoquant Pty Ltd., Adelaide, AU;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6851 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6851 (2013.01);
Abstract

Aspects of the present invention relate to methods of assessing the amplification efficiency of a primer especially the relative or absolute efficiency of a primer used in quantitative PCR amplification. Some of the methods described herein involve the assessment of primer efficiency relative to the Ct determined for a single target DNA molecule rather than Ct regression curve analysis of serially diluted samples. By assessing primer efficiency utilizing the approaches described herein, one can more efficiently select primers for the diagnosis and/or monitoring of disease conditions, analysis of specific gene regions of interest, the monitoring of disease conditions that are characterised by clonal lymphoid cell populations or disease conditions that are characterised by specific V(D)J recombination events including the detection of minimal or residual disease in leukaemia patients.


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