The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 10, 2024

Filed:

Feb. 07, 2022
Applicant:

R.j. Reynolds Tobacco Company, Winston-Salem, NC (US);

Inventors:

Jerry Wayne Marshall, Stokesdale, NC (US);

Anthony Richard Gerardi, Winston-Salem, NC (US);

Assignee:

R.J. Reynolds Tobacco Company, Winston-Salem, NC (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/36 (2006.01); A01N 63/50 (2020.01); A24B 15/18 (2006.01); A24B 15/20 (2006.01); A24B 15/30 (2006.01); C12N 15/74 (2006.01); C12N 15/82 (2006.01);
U.S. Cl.
CPC ...
C12N 9/2462 (2013.01); A01N 63/50 (2020.01); A24B 15/183 (2013.01); A24B 15/20 (2013.01); A24B 15/307 (2013.01); C12N 15/746 (2013.01); C12N 15/8243 (2013.01); C12N 15/8281 (2013.01); C12Y 302/01017 (2013.01);
Abstract

Methods and compositions are provided for expressing an endolysin in a bacterium or in a tobacco plant or plant part. Expression of the endolysin can reduce the total bacterial load on the plant and in tobacco products produced from the plant. Accordingly, the level of tobacco specific nitrosamines (TSNA) in a cured tobacco product can be decreased when the tobacco leaves used in the product were harvested from a plant expressing endolysin or when bacteria modified to express endolysin is used to treat the tobacco. Thus, bacteria and tobacco plants and plant parts are provided that express an endolysin. Further provided are methods for decreasing the microbial load on a tobacco plant and in a tobacco product by applying a solution of bacteria modified to express an endolysin or by modifying a tobacco plant to express endolysins. Smokeless tobacco products including such modified tobacco plant parts are also provided.


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