The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 03, 2024

Filed:

Nov. 30, 2020
Applicants:

Sol Inc., Seoul, KR;

Government of the United States of America, As Represented BY the Secretary of Commerce, Gaithersburg, MD (US);

Inventors:

Jong Muk Lee, Seoul, KR;

Darwin R. Reyes-Hernandez, Clarksburg, MD (US);

Brian J. Nablo, Rockville, MD (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 15/1433 (2024.01); G01N 15/14 (2024.01); G01N 15/1434 (2024.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
G01N 15/1433 (2024.01); G01N 33/5041 (2013.01); G01N 2015/1452 (2013.01); G01N 2015/1472 (2013.01); G01N 2015/1488 (2013.01);
Abstract

The present disclosure relates to a real-time quantification method of cell viability through a supravital dye uptake using a lens-free imaging system. The method includes a step of incubating a sample cell in a cell culture medium, steps of detecting light penetrating the cell culture medium and identifying a boundary region of the sample cell at a preset time interval based on the detected light, a step of staining the incubated sample cell with the supravital dye, a step of detecting intensity of light penetrating the cell culture medium at a preset time interval, a step of calculating absorbance of the sample cell included in the cell culture medium at a preset time interval based on the boundary region and the detected intensity of light and a step of analyzing a viability of the sample cell based on the calculated absorbance.


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