The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 03, 2024

Filed:

Mar. 30, 2018
Applicant:

Exothera SA, Charleroi, BE;

Inventors:

José Castillo, Brussels, BE;

Vasily Medvedev, Nivelles, BE;

Assignee:

EXOTHERA SA, Charleroi, BE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/00 (2006.01); B01D 15/38 (2006.01); B01D 37/02 (2006.01); B01J 20/14 (2006.01); C07K 16/00 (2006.01); C12M 1/06 (2006.01); C12M 1/12 (2006.01); C12M 1/34 (2006.01);
U.S. Cl.
CPC ...
C12M 47/12 (2013.01); B01D 15/3809 (2013.01); B01D 37/02 (2013.01); B01J 20/14 (2013.01); C07K 16/00 (2013.01); C12M 23/06 (2013.01); C12M 25/02 (2013.01); C12M 27/02 (2013.01); C12M 41/40 (2013.01);
Abstract

A method and a kit for cell culture clarification, as well as a system or apparatus for clarifying a cell culture and purifying biomolecules of interest, are described. The cell culture comprises at least one biomolecule of interest. The method comprises adding one or more compounds to the cell culture that allow the formation of floccules of solutes and/or particulates in said cell culture harvest, adding an amount of diatomaceous earth (DE) to said cell culture harvest, agitating the obtained solution, and transferring said solution to a filtration vessel comprising a support filter having a surface, whereby the diatomaceous earth in said solution forms a layer on said surface and filtering said harvest solution through said layer and support filter.


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