The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 26, 2024

Filed:

Jan. 04, 2021
Applicant:

Jellox Biotech Inc., Hsinchu, TW;

Inventors:

Ann-Shyn Chiang, Hsinchu, TW;

Dah-Tsyr Chang, Hsinchu, TW;

I-Ching Wang, Hsinchu, TW;

Jia-Ling Yang, Hsinchu, TW;

Shun-Chi Wu, Hsinchu, TW;

Yen-Yin Lin, Hsinchu, TW;

Yu-Chieh Lin, Hsinchu, TW;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 1/30 (2006.01); G01N 21/64 (2006.01); G01N 33/483 (2006.01);
U.S. Cl.
CPC ...
G01N 1/30 (2013.01); G01N 21/6428 (2013.01); G01N 21/6458 (2013.01); G01N 33/4833 (2013.01); G01N 2001/305 (2013.01); G01N 2021/6439 (2013.01);
Abstract

Provided is a method for preparing a tissue section, including treating a tissue specimen with a clearing agent and at least one labeling agent to obtain a cleared and labeled tissue specimen; generating a three-dimensional (3D) image of the cleared and labeled tissue specimen; performing an image slicing procedure on the 3D image to generate a plurality of two-dimensional (2D) images; identifying a target 2D image among the plurality of 2D images to obtain a distance value of D1, which indicates the distance between the target 2D image and a predetermined surface of the 3D image; preparing a hardened tissue specimen from the cleared and labeled tissue specimen; and cutting the hardened tissue specimen near a predetermined site to obtain a tissue section, wherein the distance between the predetermined site and a surface of the hardened tissue specimen corresponding to the predetermined surface of the 3D image is D1.


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