The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 26, 2024

Filed:

Sep. 26, 2019
Applicant:

Diagnologix, Llc, San Diego, CA (US);

Inventors:

Guixin Shi, San Diego, CA (US);

Kimberly Liu, San Diego, CA (US);

Ying-Ting Wang, San Diego, CA (US);

Assignee:

DIAGNOLOGIX, LLC, San Diego, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/0783 (2010.01); C12M 1/00 (2006.01); C12N 5/00 (2006.01); G01N 33/536 (2006.01); G01N 33/538 (2006.01); G01N 33/543 (2006.01); G01N 33/544 (2006.01); G01N 33/569 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0636 (2013.01); C12M 47/02 (2013.01); C12N 5/0081 (2013.01); C12N 5/0087 (2013.01); C12N 5/0093 (2013.01); G01N 33/536 (2013.01); G01N 33/538 (2013.01); G01N 33/5432 (2013.01); G01N 33/544 (2013.01); G01N 33/56972 (2013.01); C12N 2501/50 (2013.01); C12N 2501/515 (2013.01); C12N 2501/599 (2013.01);
Abstract

A method for activating and expanding isolated T cells, the method including adding to isolated T cells ligands presenting microbubbles having a flexible lipid shell with an inner bubble wall enclosing a gas and an outer bubble wall conjugated to ligands capable of achieving cell contact dependent juxtacrine signaling on the isolated T cells; and adding to isolated T cells ligands presenting microbubbles having a flexible lipid shell with an inner bubble wall enclosing a gas and an outer bubble wall conjugated to an antigen capable of forming an immunological synapse (IS) with the T cells.


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