The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 12, 2024

Filed:

Jun. 13, 2019
Applicant:

University of Washington, Seattle, WA (US);

Inventors:

Alshakim Nelson, Seattle, WA (US);

Siyami Millik, Seattle, WA (US);

Patrick Smith, Seattle, WA (US);

Dylan Karis, Seattle, WA (US);

Ashleigh Theberge, Seattle, WA (US);

Ashley Dostie, Seattle, WA (US);

Assignee:

University of Washington, Seattle, WA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B29C 48/09 (2019.01); B29C 35/08 (2006.01); B29C 48/05 (2019.01); B29C 48/16 (2019.01); B29C 48/30 (2019.01); B29C 48/475 (2019.01); B29C 48/88 (2019.01); C12M 1/12 (2006.01); B29K 77/00 (2006.01); B29K 105/00 (2006.01); B29K 105/24 (2006.01);
U.S. Cl.
CPC ...
B29C 48/09 (2019.02); B29C 35/0805 (2013.01); B29C 48/05 (2019.02); B29C 48/16 (2019.02); B29C 48/30 (2019.02); B29C 48/475 (2019.02); B29C 48/912 (2019.02); C12M 23/06 (2013.01); C12M 25/14 (2013.01); B29C 2035/0827 (2013.01); B29K 2077/00 (2013.01); B29K 2105/0061 (2013.01); B29K 2105/243 (2013.01); B29K 2105/258 (2013.01);
Abstract

Methods of fabricating a hydrogel tube, and related systems, employ extrusion of a cross-linkable hydrogel solution from an annular outer nozzle of a nozzle assembly to form a cross-linkable hydrogel tube. The cross-linkable hydrogel tube is cured to form a cross-linked hydrogel tube. A second hydrogel solution can be coextruded via the axial inner nozzle to form an inner hydrogel filament coaxially positioned within the cross-linkable hydrogel tube. The cross-linked hydrogel tube can be functionalized with collagen to enable cell adhesion, and cells can be cultured on the luminal surfaces of these tubes to yield tubular endothelial layers. A 3D printed coaxial nozzle can be used to fabricate biofunctional tubular conduits that can be utilized for engineering in vitro models of tubular biological structures.


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