The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 05, 2024
Filed:
Mar. 25, 2024
Applicant:
Northwest University, Xi'an, CN;
Inventors:
Assignee:
Northwest university, Xi'an, CN;
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/10 (2006.01); C12N 15/70 (2006.01); C12P 17/06 (2006.01);
U.S. Cl.
CPC ...
C12N 9/1051 (2013.01); C12N 15/70 (2013.01); C12P 17/06 (2013.01); C12Y 204/01 (2013.01);
Abstract
A glycosyltransferase BS-YjiC mutant, a construction method and an application thereof provided, relating to the field of genetic engineering technologies. The glycosyltransferase BS-YjiC mutant is obtained by performing site-directed mutation on amino acids at the 125, 178, 313, 125and 178, or 125and 313positions of a wild-type glycosyltransferase BS-YjiC with the amino acid sequence as shown in SEQ ID NO: 1. Compared with the wild-type glycosyltransferase BS-YjiC, the glycosyltransferase BS-YjiC mutant is more suitable for catalyzing protopanaxadiol (PPD) to generate rare ginsenosides F12 and Rh2, which is more conducive to the flexibility of the production process.