logo

For the Inventor, By the Inventor

The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 12131893 B1

PDFFull Text
Date of Patent:
Oct. 29, 2024

Filed:

Oct. 30, 2023

Method of identifying tobacco carbonyl components using non-targeted mass spectrometry

Applicant:

China Tobacco Yunnan Industrial Co., Ltd., Kunming, CN;

Inventors:

Ruizhi Zhu, Kunming, CN;

Xiaoxi Si, Kunming, CN;

Yanqun Xu, Kunming, CN;

Zhihua Liu, Kunming, CN;

Ji Yang, Kunming, CN;

Wei Jiang, Kunming, CN;

Chunbo Liu, Kunming, CN;

Fengmei Zhang, Kunming, CN;

Zhenjie Li, Kunming, CN;

Shiyun Tang, Kunming, CN;

Assignee:

CHINA TOBACCO YUNNAN INDUSTRIAL CO., LTD., Kunming, CN;

Attorneys:

Andrew D. Fortney

Central California IP Group, P.C.

Primary Examiner:

Rebecca M Fritchman

Int. Cl.
CPC ...
G01N 30/86 (2006.01); G01N 1/10 (2006.01); G16C 20/20 (2019.01); H01J 49/00 (2006.01); H01J 49/42 (2006.01); G01N 30/02 (2006.01); G16C 20/30 (2019.01);
U.S. Cl.
CPC ...
H01J 49/0036 (2013.01); G01N 1/10 (2013.01); G01N 30/8631 (2013.01); G01N 30/8679 (2013.01); H01J 49/425 (2013.01); G01N 2001/1006 (2013.01); G01N 2030/027 (2013.01); G16C 20/20 (2019.02); G16C 20/30 (2019.02);
Abstract

A method of identifying tobacco carbonyl components using non-targeted mass spectrometry is disclosed, which includes the following steps: (1) preparing a tobacco sample extract; (2) derivatizing (part of) the sample/extract with 2,4-dinitrophenylhydrazine (DNPH); (3) derivatizing (another part of) the sample/extract with DNPH-d; (4) mixing the derivatized tobacco samples; (5) preparing blank samples; (6) performing ultra performance liquid chromatography (UPLC) and ion trap (Orbitrap) high resolution mass spectrometry (HRMS) on the (derivatized) tobacco samples; (7) processing the LC-MS data; (8) filtering the mass spectrometric characteristic data to retain final chromatographic peaks; and (9) structurally annotating or identifying the retained final chromatographic peaks to obtain the tobacco carbonyl components. The method quickly removes noise or interfering components in the original data set by multiple filtering of the mass spectrometry characteristic data, and enables obtaining composition information of aldehyde and ketone chemical components in cigarettes.

Find Patent Forward Citations

Loading…