The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 29, 2024

Filed:

Oct. 30, 2017
Applicant:

Peking University, Beijing, CN;

Inventors:

Xuefeng Guo, Beijing, CN;

Xiaolong Wang, Beijing, CN;

Haina Ci, Beijing, CN;

Assignee:

PEKING UNIVERSITY, Beijing, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/34 (2006.01); C12N 9/12 (2006.01); C12Q 1/6876 (2018.01); H10K 85/40 (2023.01); B82Y 15/00 (2011.01); B82Y 30/00 (2011.01); B82Y 35/00 (2011.01);
U.S. Cl.
CPC ...
C12Q 1/6876 (2013.01); C12N 9/1252 (2013.01); H10K 85/40 (2023.02); B82Y 15/00 (2013.01); B82Y 30/00 (2013.01); B82Y 35/00 (2013.01); C12Y 207/07007 (2013.01);
Abstract

Disclosed is a DNA sequencing method. The DNA sequencing method of this invention comprises: (1) adding a tag sequence at the 3' terminus of the DNA to be sequenced so as to form a DNA to be sequenced including the tag sequence, the nucleotide sequence of said tag sequence being the reverse complement of the nucleotide sequence of the sequencing primer; (2) mixing the DNA to be sequenced including the tag sequence and the sequencing primer so as to form a product having a 5′ terminus double strand and a single main strand; (3) after step (2) is completed, the product is mixed separately with dATP, dCTP, dTTP, and dGTP to obtain four systems, each system is separately added to a single-molecule device modified by a DNA polymerase, and electric signals are read. Experiments verify that the method of this invention performs DNA sequencing and has important application value.


Find Patent Forward Citations

Guo, Xuefeng. (2024). Dna sequencing method (U.S. Patent No. 12129520). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/12129520

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