The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 29, 2024

Filed:

Jan. 18, 2022
Applicant:

Juno Therapeutics Gmbh, Munich, DE;

Inventors:

Keenan Bashour, Seattle, WA (US);

Semih U. Tareen, Seattle, WA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/86 (2006.01); C07K 14/315 (2006.01); C07K 14/725 (2006.01); C07K 16/28 (2006.01); C12N 5/0783 (2010.01);
U.S. Cl.
CPC ...
C12N 15/86 (2013.01); C07K 14/315 (2013.01); C07K 14/7051 (2013.01); C07K 16/2809 (2013.01); C07K 16/2818 (2013.01); C12N 5/0636 (2013.01); C07K 2317/55 (2013.01); C07K 2319/00 (2013.01); C12N 2500/32 (2013.01); C12N 2501/515 (2013.01); C12N 2740/10043 (2013.01);
Abstract

Provided herein are methods for transducing a plurality of cells in a composition of cells, such as a population of lymphocytes, containing viral particles. In some aspects, provided methods and reagents for the transduction of cell populations involve binding of agents to a molecule on the surface of the cells. In some cases, the reagents are multimerization reagents and the one or more agents are multimerized by reversibly binding to the reagent. In some aspects, the multimerized agent can provide for transduction and/or expansion or proliferation or other stimulation of a population of cells, and then such agents can be removed by disruption of the reversible bond. Also provided are compositions, apparatus and methods of use thereof.


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