High-affinity peptide for tumor necrosis factor alpha and application thereof

Abstract

Peptide with high binding affinity for tumor necrosis factor alpha (TNF-α). The peptide has an amino acid sequence of SEQ.ID.NO.1, or the peptide is a tandem or branched peptide with a single repeat or multiple repeats of SEQ.ID.NO.1 and SEQ.ID.NO.3 and has an amino acid sequence of SEQ.ID.NO.4. The peptide can bind to TNF-α with high affinity and can antagonize TNF-α function. When being directly injected to an animal, the peptide can significantly reduce the degree of inflammation of an animal body and improve the resistance of the animal body on an inflammatory damage. The peptide can be used for developing TNF-α antagonist drugs for treating various acute and chronic inflammatory damage, such as inflammatory, autoimmune and stress damage caused by physical, chemical and biological factors. Thus, the peptide has an extremely wide application prospect. Moreover, the peptide has a small molecular weight and low immunogenicity, and is easy to synthesize, thereby avoiding the side effects and disadvantages of traditional monoclonal antibody drug antagonists.