The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 22, 2024

Filed:

Dec. 22, 2020
Applicant:

Cepheid, Sunnyvale, CA (US);

Inventors:

Joseph H. Smith, Henderson, NV (US);

David H. Persing, San Martin, CA (US);

Alan Wortman, Redwood City, CA (US);

Ronald Chang, Redwood City, CA (US);

David Swenson, Santa Clara, CA (US);

Assignee:

Cepheid, Sunnyvale, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/686 (2018.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); C12Q 1/6848 (2018.01); C12Q 1/6851 (2018.01); C12Q 1/689 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/686 (2013.01); B01L 3/502738 (2013.01); B01L 7/52 (2013.01); C12Q 1/6848 (2013.01); C12Q 1/6851 (2013.01); C12Q 1/689 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0864 (2013.01); B01L 2400/0644 (2013.01);
Abstract

The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.


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