The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 22, 2024

Filed:

Jun. 14, 2022
Applicant:

Leica Mikrosysteme Gmbh, Vienna, AT;

Inventors:

Julia König, Vienna, AT;

Paul Wurzinger, Deutsch-Wagram, AT;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/02 (2006.01); G01N 1/28 (2006.01); G01N 1/42 (2006.01); G01N 21/64 (2006.01); G01N 23/04 (2018.01); G01N 23/06 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/02 (2013.01); G01N 1/286 (2013.01); G01N 1/42 (2013.01); G01N 21/6458 (2013.01); G01N 23/04 (2013.01); G01N 23/06 (2013.01); G01N 2001/2873 (2013.01); G01N 2223/04 (2013.01); G01N 2223/418 (2013.01);
Abstract

A method () for microscopic examination of a sample () includes applying () the sample () to a sample holder () having a transparent carrier material, capturing () a first image () of the sample () applied to the sample holder () using a first light-microscopy method, cryofixing, freeze-substituting, and subsequently infiltrating and embedding () the sample () together with the sample holder () with an embedding medium () in an embedding mold (), curing () the embedding medium (), removing the sample () from the embedding mold () together with the embedding medium () and the sample holder (), capturing () a second image () of the sample () embedded in the cured embedding medium () using a second light-microscopy method, wherein at least partially identical regions of the sample () are captured in the first and second images, and identifying () at least one portion of the first image () and one portion of the second image () which show identical regions of the sample ().


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