The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 15, 2024

Filed:

Jul. 03, 2018
Applicant:

Trustees of Tufts College, Medford, MA (US);

Inventors:

Charles R. Mace, Medford, MA (US);

Christopher Luby, Medford, MA (US);

Assignee:

Trustees of Tufts College, Medford, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01D 21/26 (2006.01); B04B 5/04 (2006.01); C12N 1/00 (2006.01); C12N 5/00 (2006.01); G01N 1/34 (2006.01); G01N 1/40 (2006.01);
U.S. Cl.
CPC ...
B01D 21/262 (2013.01); B04B 5/0414 (2013.01); B04B 5/0442 (2013.01); C12N 1/00 (2013.01); C12N 5/0081 (2013.01); G01N 1/34 (2013.01); G01N 1/4077 (2013.01); G01N 2001/4061 (2013.01); G01N 2001/4083 (2013.01);
Abstract

A method is directed to separating contaminants from mammalian cells in an aqueous multiphase system, and includes loading a container with a liquid having a top liquid phase and a bottom liquid phase. A cover medium is inserted in the container with a mixture of cultured mammalian cells and contaminants. The container is incubated and centrifuged for respective periods of time. In response to the centrifuging, the cells are separated from the contaminants in accordance with the respective density of the cells, the contaminants, and each liquid phase, resulting in the cells being located at a liquid-to-liquid interface between the top liquid phase and the bottom liquid phase and the contaminants being located at the bottom of the container.


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