The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 08, 2024
Filed:
Jul. 13, 2018
Applicant:
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, CN;
Inventors:
Jiaqi Wang, Beijing, CN;
Shengguo Zhao, Beijing, CN;
Nan Zheng, Beijing, CN;
Xu Zhou, Beijing, CN;
Songli Li, Beijing, CN;
Assignee:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/686 (2018.01); C12N 15/10 (2006.01); C12Q 1/6806 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/686 (2013.01); C12N 15/1003 (2013.01); C12Q 1/6806 (2013.01);
Abstract
In a method for extracting microbial DNA from milk, the extracted DNA can be directly used for PCR amplification. The method includes the following steps: treating milk with TE solution, fully disrupting microbial cells by bead milling, immobilizing proteins in the middle layer between the aqueous and organic phases with high-vacuum silicone grease so as to prevent impurities in the middle layer from entering the aqueous phase, etc. The extracted DNA has a high degree of intactness, high concentration and purity.