The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 01, 2024
Filed:
Mar. 30, 2023
University of Utah Research Foundation, Salt Lake City, UT (US);
Carmen Dunbar, Salt Lake City, UT (US);
Amol O. Bajaj, Salt Lake City, UT (US);
Mark M. Kushnir, Salt Lake City, UT (US);
UNIVERSITY OF UTAH RESEARCH FOUNDATION, Salt Lake City, UT (US);
Abstract
Disclosed herein are methods of determining a concentration of IGF-1 and/or IGF-2 with improved sensitivity using high performance liquid chromatography (HPLC) and mass spectrometry (MS). The methods can achieve improved sensitivity by using a supercharging reagent as part of a mobile phase of the HPLC, which can increase the amount of specific charge states of IGF-1 and/or IGF-2 ions detected by MS. An example method includes subjecting a sample to HPLC, wherein the HPLC comprises a mobile phase including a supercharging reagent and an organic acid; ionizing the sample to produce one or more ions detectable by mass spectrometry; determining amounts of IGF-1 ions in an 8charge state by mass spectrometry; and relating the amount of the determined IGF-1 ions in the 8charge state to the concentration of IGF-1 in the sample.