The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 01, 2024

Filed:

May. 04, 2021
Applicant:

Zymochem, Inc., Alameda, CA (US);

Inventor:

Harshal Chokhawala, Castro Valley, CA (US);

Assignee:

ZYMOCHEM, INC., San Leandro, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 7/42 (2006.01); C12N 9/00 (2006.01); C12N 9/02 (2006.01); C12N 9/04 (2006.01); C12N 9/06 (2006.01); C12N 9/10 (2006.01); C12N 9/12 (2006.01); C12N 9/16 (2006.01); C12N 9/24 (2006.01); C12N 9/88 (2006.01); C12N 15/52 (2006.01); C12P 7/04 (2006.01); C12P 7/18 (2006.01); C12P 7/24 (2006.01); C12P 7/40 (2006.01); C12P 7/44 (2006.01); C12P 7/6409 (2022.01); C12P 13/00 (2006.01); C12P 17/08 (2006.01); C12P 17/10 (2006.01);
U.S. Cl.
CPC ...
C12N 15/52 (2013.01); C12N 9/00 (2013.01); C12N 9/0006 (2013.01); C12N 9/0008 (2013.01); C12N 9/001 (2013.01); C12N 9/0016 (2013.01); C12N 9/1029 (2013.01); C12N 9/1096 (2013.01); C12N 9/1205 (2013.01); C12N 9/13 (2013.01); C12N 9/16 (2013.01); C12N 9/2402 (2013.01); C12N 9/88 (2013.01); C12N 9/93 (2013.01); C12P 7/04 (2013.01); C12P 7/18 (2013.01); C12P 7/24 (2013.01); C12P 7/40 (2013.01); C12P 7/42 (2013.01); C12P 7/44 (2013.01); C12P 7/6409 (2013.01); C12P 13/001 (2013.01); C12P 13/005 (2013.01); C12P 17/08 (2013.01); C12P 17/10 (2013.01); C12Y 101/01001 (2013.01); C12Y 101/01002 (2013.01); C12Y 101/01035 (2013.01); C12Y 101/01078 (2013.01); C12Y 101/01268 (2013.01); C12Y 101/01269 (2013.01); C12Y 102/01003 (2013.01); C12Y 102/01005 (2013.01); C12Y 102/01022 (2013.01); C12Y 102/01024 (2013.01); C12Y 102/01026 (2013.01); C12Y 102/01048 (2013.01); C12Y 102/01063 (2013.01); C12Y 102/07005 (2013.01); C12Y 102/99006 (2013.01); C12Y 103/01044 (2013.01); C12Y 103/01045 (2013.01); C12Y 103/01086 (2013.01); C12Y 104/01 (2013.01); C12Y 203/01001 (2013.01); C12Y 203/01032 (2013.01); C12Y 203/01035 (2013.01); C12Y 203/01057 (2013.01); C12Y 206/01008 (2013.01); C12Y 206/01009 (2013.01); C12Y 206/01036 (2013.01); C12Y 206/01043 (2013.01); C12Y 206/01048 (2013.01); C12Y 206/01076 (2013.01); C12Y 206/01082 (2013.01); C12Y 207/01031 (2013.01); C12Y 207/01165 (2013.01); C12Y 208/03 (2013.01); C12Y 301/03 (2013.01); C12Y 301/03002 (2013.01); C12Y 301/03008 (2013.01); C12Y 301/03019 (2013.01); C12Y 301/0302 (2013.01); C12Y 302/01 (2013.01); C12Y 401/01001 (2013.01); C12Y 401/02 (2013.01); C12Y 401/02014 (2013.01); C12Y 401/0202 (2013.01); C12Y 401/02021 (2013.01); C12Y 401/03016 (2013.01); C12Y 401/03017 (2013.01); C12Y 402/01002 (2013.01); C12Y 402/01003 (2013.01); C12Y 402/01028 (2013.01); C12Y 402/0103 (2013.01); C12Y 402/01079 (2013.01); C12Y 402/0112 (2013.01); C12Y 602/01 (2013.01); Y02E 50/30 (2013.01);
Abstract

Provided herein are methods, compositions, and non-naturally occurring microbial organism for preparing compounds such as1-butanol, butyric acid, succinic acid, 1,4-butanediol, 1-pentanol, pentanoic acid, glutaric acid, 1,5-pentanediol, 1-hexanol, hexanoic acid, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, ε-Caprolactone, 6-amino-hexanoic acid, ε-Caprolactam, hexamethylenediamine, linear fatty acids and linear fatty alcohols that are between 7-25 carbons long, linear alkanes and linear α-alkenes that are between 6-24 carbons long, sebacic acid and dodecanedioic acid comprising: a) converting a Caldehyde and pyruvate to a Cβ-hydroxyketone intermediate through an aldol addition; and b) converting the Cβ-hydroxyketone intermediate to the compounds through enzymatic steps, or a combination of enzymatic and chemical steps.


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